Recombinant adenovirus (Ad) is a replication-defective adenovirus vector system. Adenovirus packaging relies on cell lines expressing E1 genes, such as HEK-293 and HEK-293A, as the adenoviral packaging is defective in the early transcription of replication genes E1 and E3 (DE1, DE3), with the E3 gene being non-essential for viral production.

The widely utilized Adenovirus vectors are predominantly derived from human adenovirus type 5 (Ad5), featuring a linear double-stranded DNA genome approximately 36 Kb in length. Ad5 vectors offer several notable advantages: they possess a broad infection range, with nearly all cell lines and tissues susceptible to infection, achieving efficiencies as high as 100%. This efficacy surpasses that of other viral vector tools and liposome transfection methods. Additionally, Ad5 vectors do not integrate their genome into host cell genes, mitigating the risks associated with gene mutation and integration. As a result, recombinant adenovirus finds extensive applications in gene therapy, fundamental life science research, and various other fields.

Gene Universal's adeasy adenovirus packaging system (Ad5 type) offers distinct advantages compared to the admax system. Notably, it facilitates easier detection of false positives of linearized plasmid recombination in the pronucleus. Additionally, the linearized adenovirus plasmid can be utilized in cell lines expressing E1, such as HEK-293 and HEK-293A. Our adenovirus vectors meet the rigorous requirements for both cell infection and in vivo experiments, ensuring reliable results for your research endeavors.