The principle of action of UDG enzyme is to selectively hydrolyze and break the uridine glycosidic bond of double-stranded or single-stranded DNA containing dU. Replace dTTP with dUTP, so that all PCR products contain dU DNA strands. Adding an incubation step before the start of PCR, the UDG enzyme can degrade the uracil bases in the existing U-DNA pollutants in the reaction system, and break the DNA strands under subsequent denaturing conditions, eliminating the pollution caused by aerosols Amplification, so as to ensure the specificity and accuracy of the amplification results. The UDG enzyme was inactivated at the high temperature of 95°C at the beginning of PCR, and it would no longer degrade the newly amplified product U-DNA.
PCR reaction is prepared according to the following system
Components | Volume(ul) | Final Conc. |
---|---|---|
2×GUeasy Hot start Taq probe qPCR Master Mix | 10 | 1× |
Primer Mix(10uM) | N | 0.1-0.5uM |
Probe Mix(10uM) | N | 50-250nM |
UDG enzyme(1U/ul) | 1 | 0.04U/ul |
Template | 1-5 | - |
ddH2O | up to 20ul | - |
Product | Catelog# | Size | Activity | Price |
---|---|---|---|---|
Uracil-DNA Glycosylase | U3004-02 | 200ul | 200U | Inquiry |
U3004-05 | 500ul | 500U | Inquiry | |
U3004-1 | 1ml | 1000U | Inquiry | |
U3004-3 | 3ml | 3000U | Inquiry | |
U3004-5 | 5ml | 5000U | Inquiry |
* More discount is available for bulk order
Shipped in ice-pack, stored at -20°C, valid for two years.
To meet your needs to the fullest extent, Gene Universal also provides Molecular Cloning and DNA Marker Product、 Protease Products、 Competent Cell Products.
Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.
Order form Download To ensure a successful upload of your order, pls download the latest forms.
Phone: +1 302-235-9792
Fax:+1 302-355-3499