Featured Synthetic DNA Libraries

1. Site-directed Mutagenesis Library: Site-directed mutagenesis library involves sequentially substituting a customer-determined amino acid position with all 19 other amino acids to generate a mutant library. This method enables scientists to conduct structural-functional analysis, examining the impacts of specific amino acid substitutions, both beneficial and adverse.

2. Scanning Library: Scanning Library involves sequentially substituting specific residues within a customer-defined region with one specified residue via site-directed mutagenesis. Alanine and Cysteine scanning are two commonly utilized types of scanning libraries.

3. Randomized Library: A randomized mutant library enables random amino acid substitutions within a user-defined region (e.g., ORF region within 200bp-1500bp). These proteins can be expressed and assayed for function to identify beneficial substitutions in protein engineering studies.

4. Degenerated Library: Scientists can create a library of mutants by combining various genetic elements in multiple combinations. Gene Universal provides both traditional partially randomized (NNK/NNS) and completely randomized (NNN) mutant library services to support this endeavor.

5. Truncation Library: A truncation library of mutants is constructed by trimming amino acids from either the N- or C-terminus (or both) of a protein around a specified core region. This enables scientists to pinpoint the core region responsible for protein function.